Three bacteria, E.coli, Staphylococcus epidermis, and Micrococcus luteus, have been grown on TSI or Triple sugar iron agar medium, which is a . Enterococcus spp. It can be easily be mistaken for staphylococci, as they are very similar morphologically and physiologically (Wikipedia, Staphlyococcus Aureus). . After you have cultivated your organism on agar media, the next step is the microscopic examination. The categorization of the Gram-positive cocci based on their possession of the enzyme catalase, a quality which can be easily assayed in the lab. Micrococcus luteus Grown on BrainHeart Infusion Agar, Klebsiella characteristics on MacConkey Agar, Clinical Case Leukocyte Vacoulation Bacterial Infection, Segmented neutrophilic granulocyte during degradation, DIC (Disseminated intravascular coagulation), Creatinine Phosphate Kinase (CPK) and CK-MB Overview. Micrococcus luteus has been shown to survive in oligotrophic environments for extended periods of time. It shows that the sample contains bacteria from the Terrabacteria group. Micrococcus lylaeare Gram-positive cocci mostly arranged in tetrads. Micrococcus luteus is coagulase negative, bacitracin susceptible, and forms bright yellow colonies on nutrient agar. Micrococcus spp. Further tests that I would do would be testing how much heat resistance it has, the density of a broth suspended sample using a dilution series, test for more antibiotic resistances, and how well it can grow in antibacterial mediums and mediums of different pH levels. 2. Finally, when looking at the API 20E strip none of the results returned positive despite the culture being active and fresh from being streaked recently. Micrococcus species occur in a wide range of environments, including dust, water, and soil. They are found in soil, dust, water and air, and as part of the normal microbiota of skin. Annals of Allergy, Asthma and Immunology 122(1):17-24. luteus is capable of surviving in environments Your email address will not be published. Staphylococcus and Micrococcus: Introduction, Differentiating Fea Your email address will not be published. These discrepancies could be due to human error, unpure culture, or an old agar plate. If acid is produced, the pH indicator will turn yellow. Micrococcus Catalase Test: The catalase test facilitates the detection of the enzyme catalase in bacteria. Each family of Gram-positive cocci has its own unique configuration. 2019). Micrococcus are aerobic, Gram-positive cocci ranging in size from 0.5 to 2.0 m in diameter. They grow in circular, entire, convex, and usually non-pigmented or cream white colonies with diameters of approximately 4 mm after 2-3 days on the plate at 37C. The modified oxidase reagent is prepared as tetramethyl-p-phenylenediamine in certified grade dimethyl sulfoxide. The majority of staphylococcal species produce non-pigmented colonies with smooth margins, as opposed to the important pathogen, Staphylococcus aureus, which produces the characteristic colonies seen here. [3] Its genome was sequenced in 2010 and is one of the smallest genomes of free-living Actinomycetota sequenced to date, comprising a single circular chromosome of 2,501,097 bp.[4]. Micrococcus varians Micrococcus luteus Staphylococcus saprophyticus Staphylococcus epidermidis Streptococcus pneumoniae Streptococcus mitis Biochemical Tests LAB REPORT.docx - Biochemical Tests in Micrococcus luteus growth on nutrient agar is bright mustard-yellow colonies as shown above image. This is termed alpha () hemolysis and results in a greenish color surrounding the colony. The API 20 E test strip results revealed nothing about what the isolate uses as an electron acceptor because every result was negative, and that it likely only uses glucose as an energy and carbon source and oxygen as its electron acceptor.
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